Figure 1

TEM analysis of particles incubated at (A) pH 7.4 and (B) pH 5.5 (scale bar 200 nm). (C) FT-IR spectra of free Lf (black), LfNPs (green), TMZ (blue) and TMZ-LfNPs (red). (D) Release of TMZ from LfNPs studied at different pH values. pH 5.5 and pH 7.4 are highlighted in grey and black respectively. (E) Cumulative TMZ release from LfNPs at pH 5.5 (grey) and pH 7.4 (black). Each data points were repeated in triplicates (n = 3) and presented as Mean ± Standard Deviation (S.D).