Figure 6

RNAseq analysis of transcriptome of wild-type and MZikk2 ^−/− embryos (obtained from homozygotic parents) reveals novel molecular targets regulated by Ikk2. (A–D) Green - down regulated in ikk2 ^−/− as compared to wild type control, red - up regulated in ikk2 ^−/− as compared to wild type control. (A) Heat maps illustrating total stage- and gene-specific expression trends. (B) Comparison of expression of angiogenesis-related genes. (C) Comparison of expression of skin-related genes. (D) Comparison of expression of cytokinesis-related genes. (E) Real-time PCR (qPCR) verification of selected target genes identified by RNAseq. Two internal reference, actb (actin) and gapdh were used for normalization. Data quantification was done by using the ΔΔCt method and the result was presented as a relative fold change compared to wild-type embryos at 2 and 4 hpf. Data are expressed as mean ± SEM, p < 0.05 for all four genes listed.