Figure 5

CDDO-DFPA-exposed DCs suppress Th17 induction via soluble mediators. BMDCs were pretreated in the presence or absence of CDDO-DFPA (100–400 nM) for 1 hour and washed before incubation with LPS (100 ng/ml) for 24 hours. DC conditioned medium was collected and stored in −80 °C. Splenic CD4+ T cells were isolated from C57BL/6 mice and incubated under the indicated conditions with the addition of DC-cultured medium. T cells were stimulated for 2 days (Th17) or 3 days (Treg) with plate-bound CD3 and CD28 antibodies and in the presence of differentiation factors (TGF-β and IL-6 for Th17, TGF-β only for Treg). Subsets of IL-17+ Th17 (A) and CD25+/Foxp3+ Treg (B) were analyzed by flow cytometry. Similar results were obtained in three independent experiments.