Figure 6 | Scientific Reports

Figure 6

From: A unique tolerizing dendritic cell phenotype induced by the synthetic triterpenoid CDDO-DFPA (RTA-408) is protective against EAE

Figure 6

CDDO-DFPA increased Treg and suppressed Th1 and Th17 responses during EAE induction. (A) Mice were immunized with MOG (35–55) and treated with either vehicle control or CDDO-DFPA daily from day 3 to day 15 by i.p injection. Mice were sacrificed at day 17 and splenocytes were harvested and stimulated with PMA/ionomycin/Golgistop for 4 hours and subjected to flow cytometry to determine the frequency of Th1, Th2, Th17, and Treg subsets among CD4+ T cells based on their expression of IFN-γ, IL-4, IL-17, and Foxp3, respectively. (B) Quantification of data was presented as the mean ± S.E.M. (n = 4–5 mice in each group). *P < 0.05, ***P < 0.001, One-way ANOVA with the Bonferroni corrections.

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