Figure 7
Passively DC-induced EAE is abrogated by DC exposure to CDDO-DFPA. (A) EAE induction by MOG (35–55)-pulsed BMDCs. Mature BMDCs were treated in the presence or absence of CDDO-DFPA (400 nM) and subsequently pulsed with MOG (35–55) for 4 hours. A total of 200 μl of 2 × 106 cells were administered by subcutaneous injected into the flank region of C57BL/6 mice once each week for a total of four injections. Each time, PTX was administered by i.p. injection immediately and again 2 days later (day 0 on 4th cycle). (B) Clinical scores were recorded after all four injections, using standard criteria. All data were presented as the mean ± S.E.M. *P < 0.05. Multiple t-tests with Holm-Sidak analysis (n = 7 mice in each group). (C and D) These mice were sacrificed on day 17 and mRNA expression of IFN-γ and IL-17 in the spleen (C) and IFN-γ mRNA expression in the lumbar spinal cord were measured by qRT-PCR. The results in panel C and D were presented as the mean ± S.E.M. (n = 3–6 mice in each group). *P < 0.05, **P < 0.01, One-way ANOVA with the Bonferroni corrections.
