Figure 6
From: Integrin β3/Akt signaling contributes to platelet-induced hemangioendothelioma growth
The integrin β3/Akt signaling contributed to platelet-induced EOMA cell proliferation. (a) EOMA cells were transfected with control or integrin β3 siRNA for 4 days, and then treated with platelets for another 72 hours. The cell viability was examined using the CCK8 assay. (b) The EOMA cells were incubated with indicated concentrations of Akt inhibitor GSK690693 for 72 hours. GSK690693 treatments with 1 and 2 μM did not significantly affect EOMA cell survival. (c) EOMA cells were pre-treated with Akt inhibitor GSK690693 for 3 hours, and then incubated with platelets for another 72 hours. The cell viability was examined using the CCK8 assay. (d) EOMA cells were either transfected with control or integrin β3 siRNA for 4 days, or pre-treated with GSK690693 for 3 hours, and then incubated with platelets for another 48 hours. The cell proliferation was assessed via the EdU assay. Scale bar, 60 μm. n = 3–5, one-way or two-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.
