Figure 2 | Scientific Reports

Figure 2

From: Multi-color single-molecule tracking and subtrajectory analysis for quantification of spatiotemporal dynamics and kinetics upon T cell activation

Figure 2

Usage of planar lipid bilayers for examination of molecular mobility in living cells. Single-molecule images of Qdot 585-labeled CD45 in living Jurkat cells on a lipid bilayer (A,B) and on an antibody-coated glass surface (C,D). Single-frame images (A,C) and 200-frame averaged images (B,D) recorded at 33 ms/frame are shown. The difference between single-frame images and multi-frame averaged images indicates high molecular mobility. Bar, 10 μm.

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