Figure 4

Single-molecule trajectories of CD3ε and CD45 relative to TCR microclusters on the cell membrane of a living Jurkat cell. (A) Schematic representation of trajectory durations, residence times, dissociated-state durations, and associated-state durations for lifetime and kinetics analyses. Each square indicates the diffusion coefficient of a single subtrajectory. The locations of the subtrajectories relative to the microclusters are indicated by background colors: inside (white), boundary (light gray), and outside (gray). (B,C) Representative time courses of the diffusion coefficient D of CD3ε (B) and CD45 (C) obtained by the moving subtrajectory MSD analysis. The three diffusion types are shown as follows: simple (green square), directional (cyan square), and confined (magenta square). The squares and the background colors are the same as in (A). (D,E) Lifetimes of the residence times of CD3ε (D) and CD45 (E) on the inside and outside of the microclusters. (F) Ratio for N _{res_bou–>in}/N _{res_bou–>out} between the number of data elements of the residence times at the boundary exiting to the inside and those exiting to the outside.