Figure 1 | Scientific Reports

Figure 1

From: ShadowY: a dark yellow fluorescent protein for FLIM-based FRET measurement

Figure 1

Sequence alignment of EYFP, ShadowY, and sREACh. (a) Green rectangles indicate the differences between ShadowY and EYFP. Yellow boxes indicate the differences between ShadowY and sREACh. The chromophore tripeptide is highlighted with the magenta box. (b) X-ray crystal structure of EYFP (Protein Data Bank ID: 3V3D). The mutated amino acid positions except F46L and Q69M are indicated.

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