Table 1 Characteristics of ShadowY.

From: ShadowY: a dark yellow fluorescent protein for FLIM-based FRET measurement

Protein

EC (M−1cm−1)

QE

Ex (nm)

Em (nm)

Folding t1/2 (sec)

Oxidation t1/2 (min)

Fluorescence lifetime (ns)

purified

in cells

ShadowG

89,000*

0.005 *

486*

510*

37*

76*

0.16*

ND

sREACh

115,000*

0.07*

517

531*

267

130

0.67

0.54

sREACh (#1)

114,000

0.03

518

530

149

ND

0.37

0.33

sREACh (#2)

130,000

0.02

519

531

75

ND

0.25

0.22

ShadowY

136,000

0.01

519

531

73

140

0.19

0.17

  1. EC: extinction coefficient, QE: quantum efficiency, Ex: excitation maximum, Em: emission maximum. *Values obtained from previously published data8. sREACh#1 contains the following mutations in sREACh: N144A, N146P, S147V, A206K, and R223F. sREACh#2 contains the following mutations in sREACh#1: Q204S and S205A. ShadowY contains the following mutations in sREACh#2: K166S and R168Y. ND: not determined.
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