Table 2 Characteristics of Clover mutants.

From: ShadowY: a dark yellow fluorescent protein for FLIM-based FRET measurement

Protein

EC (M−1cm−1)

QE

Ex (nm)

Em (nm)

Fluorescence lifetime (ns)

Förster distance with

purified

in cells

ShadowY (nm)

sREACh (nm)

mEGFP

58000*

0.73*

488*

507*

2.73

2.60

6.2

6.2*

Clover

111000†

0.76†

505†

515†

3.29

3.11

6.3

6.3

CloverT153M

112000

0.80

505

515

3.34

3.11

6.4

6.4

CloverT153M/F223R

122000

0.81

505

515

3.31

3.08

6.4

6.4

  1. EC: extinction coefficient, QE: quantum efficiency, Ex: excitation maximum, Em: emission maximum. *,†Values obtained from previously published data8, 13, respectively. For the calculation of Förster distance, random interfluorophore orientation were assumed 30. ND: not determined.
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