Figure 4
Resveratrol modifies glucose utilization in colon cancer cells. (A) Caco2 cells were treated with 10 µM resveratrol (RES) for 48 hr and ATP production was estimated by using ATP Bioluminescence assay kit II. (B) After a 48 hour-treatment with 10 µM, subconfluent Caco2 cells were isolated and 106 cells were incubated in Krebs-Ringer phosphate buffer supplemented with 5 mM [14C1]-glucose (isotopic dilution 1/1000) or [14C6]-glucose (isotopic dilution 1/100). 14CO2 was recovered and pentose phosphate pathway (PPP) activity was calculated as described in the Material and Methods section. (C) After treatment with resveratrol, cells were isolated and incubated in Krebs-Ringer phosphate buffer supplemented with 5 mM [U14C]-glucose (isotopic dilution 1/1000). The incorporation of carbons from glucose was measured in total lipids according to Bligh and Dyer57 as described in the Material and Methods section. The means ± SEM are shown for at least three independent experiments performed in triplicate (**p < 0.01, ***p < 0.001 vs control).
