Figure 6
Resveratrol modulates PDP1 level in cancer cells. Caco2 cells were cultured at subconfluence in 5% charcoal-treated serum in DMEM 24 hr before and during the treatment with 10 µM resveratrol (RES) for the indicated times. (A) mRNA levels of PDK1, PDP1 and PDP2 were evaluated by real time RT-PCR and normalized using RPL13 RNA. (B) After a 48 hr exposure with resveratrol 10 µM, total cell lysates were collected using RIPA buffer supplemented with protease and phosphatase inhibitors and analyzed by Western blot using antibodies against PDK1, PDP1 and actin for normalization. Data are the means ± SEM of at least 3 independent experiments (*p < 0.05).
