Figure 4
From: Bone matrix components activate the NLRP3 inflammasome and promote osteoclast differentiation
Bone matrix components induce OC formation in co-cultures. BMSC and BMM were obtained from WT or Il-1r −/− mice, and the co-cultures were set up for various cell combinations at a 1:1 ratio. After 2 days of co-cultures, bone particles (BP) were added and TRAP staining was performed at day 4 (a), and OC number was determined by counting multinucleated, TRAP-positive cells (b). **p = 0.0084, ***p = 0.0004. Data are means ± SD of samples run in triplicates, and are representative of at least 2 independent experiments. p values correspond to significant differences relative to WT BMSC:WT BMM (control).
