Figure 3

Analysis of miR-196a and miR-196b expression and ANXA1 mRNA levels in HNSCC tissue specimens. (A) miR-196a and (B) miR-196b expression levels were quantified by RT-qPCR in 11 fresh primary tumors (T) and patient-matched normal epithelia (N). Data were normalized to RNU48 levels, and relative to the normal mucosa from non-oncologic patients. (C) ANXA1 mRNA levels were quantified by RT-qPCR and data normalized to RPL19 levels. p-values obtained from the Wilcoxon test provided statistical confirmation of the significant changes.