Figure 1 | Scientific Reports

Figure 1

From: Regulatory T-cells from pancreatic lymphnodes of patients with type-1 diabetes express increased levels of microRNA miR-125a-5p that limits CCR2 expression

Figure 1

MicroRNA expression profiles reveal that miR-125a-5p is over-expressed in Treg cells isolated from PLN of patients with T1D. (A) microRNA expressions in Treg cells isolated from peripheral blood (PB) of non-diabetic control (CTR) donors (n = 8), pancreatic lymph nodes (PLN) of controls (n = 3) and from PB and PLN of patients with T1D (n = 4) is reported in the hierarchical heatmap. Expression values are reported as delta cycle threshold (dCT) values normalized using three different small RNAs (RNU6, RNU44, RNU48) and represented by scale colour of normalized dCT values [Red- low expressed microRNAs; blue- highly expressed microRNA]. Euclidean complete linkage clustering method was used to infer the data in the heatmap clustering graph. (B) Zoom-in of heatmap graph reporting miR-125a-5p expression values [Red- low expressed microRNAs; blue- highly expressed microRNA]. (C) The expression levels of miR-125a-5p were extrapolated from the heat map shown in panel B. Dots connected by a line represent paired samples. *p < 0.05 paired 2-tailed “Student t-test” on dCt.

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