Figure 3 | Scientific Reports

Figure 3

From: Regulatory T-cells from pancreatic lymphnodes of patients with type-1 diabetes express increased levels of microRNA miR-125a-5p that limits CCR2 expression

Figure 3

TNFR2 and CCR2 are targets of microRNA miR-125a-5p. Luciferase activity in HeLa cells transfected with (A) IL6R (wt, mutated)-, (B) FOXP3-, (C) TNFR2 (wt, mutated)- or (D) CCR2-3′UTR (wt, mutated)-Firefly Luciferase reporter plasmid together with miR-125a-5p or with scrambled microRNA expressing vector. Firefly luciferase activity was divided by the Renilla luciferase activity to correct eventual differences in transfection efficiency. Bars represent mean + SD of 3 independent experiments. *p < 0.05 paired student’s t test.

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