Figure 6
Mdivi-1 decreases ROS-mediated lipid oxidation in A53T animals. (a) Representative pictures of 4-HNE staining (red) measured in TH positive neurons (green). Scale bar 30 μm. (b) Dopaminergic cells of animals expressing hA53T-α-syn display an increase of lipid oxidation that turned back to basal levels in the presence of mdivi-1 treatment. Quantification was carried out measuring the levels of grey of 4-HNE staining in 16-bit images. Fluorescent images are captured as single layer. Values are means ± SEM for 7–8 rats (6 cells are analysed for each rat) and were analysed by two-way ANOVA (AAV injection: F 1,27 = 5.196 p = 0.0308; treatment: F 1,27 = 4.014 p = 0.0552; AAV injection x treatment: F 1,27 = 16.91 p < 0.001) followed by Tukey multiple comparison test (*p < 0.05; **p < 0.01; ***p < 0.001 to A53T vehicle).
