Table 1 Structural characterization of the polysaccharide formed upon incubation of amylose V with the L. reuteri NCC 2613 GtfB-ΔN enzyme.

Parameter	Type of glucosyl units	A. chroococcum GtfD polymer^c	P. beijingensis GtfD HMM polymer^d	P. beijingensis GtfD LMM polymer^d	L. reuteri NCC 2613 GtfB-ΔN polymer
Methylation analysis (%)	Glcp(1 →	19	17	15	15
	→ 4)-Glcp-(1 →	45	54	62	59
	→ 6)-Glcp-(1 →	18	11	5	10
	→ 4,6)-Glcp-(1 →	18	18	18	16
NMR chemical shift (%)^a	(α1 → 4)	68	71	77	75
NMR chemical shift (%)^a	(α1 → 6)	32	29	23	25
Molecular mass (10³ Da)^b		13 10³	27 10³	19	7

For comparison the characteristics of the polymers produced by the A. chroococcum and P. beijingensis GtfD 4,6-α-GTases are included as well.
^aThe data represents the ratios of integration of the surface areas of the (α1 → 6) linkage signal at 4.97 ppm and the (α1 → 4) linkage signal at 5.36 ppm in the ¹H NMR spectra of the polysaccharides (see Fig. S2). ^bThe average molecular mass of polysaccharide was determined in duplicate. ^cTaken from Gangoiti et al.²⁰. ^dTaken from Gangoiti et al. ²¹.

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