Figure 1 | Scientific Reports

Figure 1

From: Integration of miRNA and mRNA expression profiles reveals microRNA-regulated networks during muscle wasting in cardiac cachexia

Figure 1

Monocrotaline-treated rats develop right heart failure and cardiac cachexia associated with muscle wasting and fiber-type changes. Representative cross-sections of soleus muscles showing fiber-type distribution using myofibrillar adenosine triphosphatase (m-ATPase) reaction after pre-incubation at pH 4.3, from control (CT) and cardiac cachexia (CC) groups (A). CC decreases cross-sectional area (CSA; µm2) of fiber-types I, Ic/IIc, and IIa (B), induces a slow-to-fast switch in fiber types composition (C), and changes the relative myosin heavy chain (MyHC) isoform percentage (%) in soleus muscle (D). Expression of atrogenes (Fbxo32 and Trim63), Myod, and slow and fast (Tnni1, Tnni2, and Tnnt3) myofiber genes in soleus muscle of CC compared to their levels of expression in CT (set at a value of 0), as detected by real-time PCR. Y-axis represents log2-fold change. The expression of each transcript was normalized by the expression of Ppib, B2m, and Ppia. (E) Data are expressed as mean ± SD; n = 6 per group. *p < 0.05; **p < 0.001: statistical significance versus CT group.

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