Figure 8 | Scientific Reports

Figure 8

From: Integration of miRNA and mRNA expression profiles reveals microRNA-regulated networks during muscle wasting in cardiac cachexia

Figure 8

miR-29b promotes muscle atrophy and reduces collagens expression. Immunofluorescence of C2C12 myotubes transfected with miR-29b-3p-mimic stained with antibodies specific to myosin heavy chain Myh2 (red). Nuclei stained with DAPI (blue) (A). Quantitative analyses of myotubes area (B). Total protein concentration in C2C12 myotubes measured by the Bradford assay (C). Number of total myotubes nuclei (three or more nucleus/cells) (D). mRNA levels of Col1a1, Col3a1, Myh7, and Myh2 in myotubes after miR-29b-mimic transfection compared to their levels of expression in CT, as detected by real-time PCR. The expression of each transcript was normalized by the expression of Ppia and Ppib (E). Data represent the average of three independent experiments and are expressed as mean ± SD. *p < 0.05; **p < 0.001: statistical significance versus CT group.

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