Figure 3 | Scientific Reports

Figure 3

From: Arsenic exposure impels CD4 commitment in thymus and suppress T cell cytokine secretion by increasing regulatory T cells

Figure 3The alternative text for this image may have been generated using AI.

Effect of arsenic exposure on splenic CD4+ T cells. Splenocytes (0.2 × 106) isolated from mice treated with arsenic (0.00, 0.038, 0.38 and 3.8 ppm) orally for 7, 15 and 30 days were stained with PE conjugated anti-CD4 monoclonal antibody and PerCP conjugated anti-CD8 monoclonal antibody. The PE and PerCP fluorescence were measured using flow cytometer. (a) Percentage of CD4+ and CD8+ T cells in splenocytes at 7, 15 and 30 days. (b) Each representative dot plot reflects splenocytes stained CD4 and CD8 antibodies. Arsenic promoted percent distribution of CD4+ T cells in spleen. Values are mean ± S.E. (n = 9 per group). *p < 0.05, **p < 0.01 and ***p < 0.001 compared to control.

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