Figure 8
Effect of arsenic exposure on general parameters. Following arsenic exposure mice body weight was recorded weekly. (a) Line graph represents the changes in body weight. Values presented as mean ± S.E at each time point (n = 5–8 per group). (b) Thymocytes and (c) splenocytes (0.1 × 106 cells/200 µl/well) isolated from mice treated with arsenic (0.00, 0.038, 0.38 and 3.8 ppm) orally for 7, 15 and 30 days were cultured at 37 °C in 5% CO2 incubator and viability was checked following 72 h incubation using Presto blue reagent. Fluorescence was measured at 544 (ex.) and 610 (em.) expressed as percent of control (n = 3–5 per group). Serum level of (d) AST, ALT and (e) urea expressed as mean ± S.E. (n = 3 per group). Representative micrographs of histo-architecture of (f) thymus and (g) spleen obtained at 10x magnification (scale bar = 60 µm) from control and 30 day exposed groups.
