Figure 1 | Scientific Reports

Figure 1

From: Femtogram Level Sensitivity achieved by Surface Engineered Silica Nanoparticles in the Early Detection of HIV Infection

Figure 1

Schematic illustration of SNIA for detection of HIV-1 p24 antigen. HIV-1 p24 is bound to capture antibody which was coated on microtiter wells and then sandwiched with secondary biotinylated anti-p24 antibody. The immunocomplex is then coupled to Streptavidin conjugated silica nanoparticles, which recognizes and binds to the above biotinylated antigen–antibody complex. After extensive washing, the fluorescence signal released from the complex is then recorded and quantified with Spectramax M5 multiplate reader.

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