Figure 5

Calibration curve of silica nanoparticle based immunoassay (SNIA) for the detection of HIV-1 p24 antigen (n = 3). Purified HIV-1 p24 antigen (range, 0.02 pg/mL to 1000 pg/mL in serial dilution in PBS) served as target. The normalized relative signal intensities are represented as the ratios of samples over the cutoff value of the negative control. The dotted line (---) represents the cutoff value of negative control. The error bars represent the standard deviation of at least three independent repeated experiments for each assay.