Figure 2 | Scientific Reports

Figure 2

From: Uracil Accumulation and Mutagenesis Dominated by Cytosine Deamination in CpG Dinucleotides in Mice Lacking UNG and SMUG1

Figure 2

UNG and SMUG1 complement each other in U excision and UNG/SMUG1 double knockout mice accumulate a large amount of genomic uracil. (a) Uracil excision activity of Ung −/− and Smug1 −/− organ extracts relative to WT in U:G, U:A, and single-stranded oligonucleotides. Biochemical assays were evaluated using two-tailed t-test 95% confidence level. (b) There was no significant genomic dU accumulation in organs from either Smug1 +/− or Smug1 −/− mice. Genomic dU increased slightly in Ung −/− and drastically in Ung −/− Smug1 −/− mouse organs, respectively. The effect of SMUG1 and UNG knockout on genomic lesion levels were assessed using an unpaired t-test or analysis of variance (ANOVA). Error bars indicate SD of three biological replicates.

Back to article page