Figure 4 | Scientific Reports

Figure 4

From: ROCK-1 mediates diabetes-induced retinal pigment epithelial and endothelial cell blebbing: Contribution to diabetic retinopathy

Figure 4

Effect of intravitreal fasudil on ROCK-1 activation state. Western blotting analyses to assess ROCK-1 protein level showed no significant change in any group with Fasudil treatment in GK rats as compared to vehicle treated GK rats (V) or WS rats (n = 6–7 per group) (a). An in vitro ROCK activity immunoblot kit evaluating recombinant MYPT1 phosphorylation level was performed and showed a decrease in ROCK activity in GK Fasudil treated rats (b) (n = 5 per group). We confirmed ROCK-1 activity inhibition by the significant decrease of MLC phosphorylation in GK Fasudil treated rats (c) (n = 12 per group); ***p value < 0.001. In GK rats, activation of ROCK-1 coincides its membrane recruitment and associated with P-MLC at the membrane (D,b,e). Fasudil restores the cytoplasmic localization of ROCK-1 and MLC (D,c,f).

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