Figure 3
From: Novel animal model defines genetic contributions for neuron-to-neuron transfer of α-synuclein
BiFC-syn C. elegans model reveals neuron-to-neuron transfer that increases with age. (A) BiFC-induced expression of EGFP detected in the head region and nerve ring, the HSN neurons and axon and the PVP axon in the tail region of the worm in 1, 5, 10 and 14 days old BiFC-syn worms. * Denotes location of vulva. (B) Quantification of the fluorescence intensity in the nerve ring and head region of 1, 5, 10 and 14 days old worms. Statistical significance was determined from the mean values of three independent experiments with at least 75 worms per group, using ANOVA in conjunction with a Tukey’s post-hoc multiple comparisons test (alpha = 0.05) (C) Relative mRNA levels of the BiFC1 construct in BiFC-syn worms and control worms expressing only the BiFC1 construct in L4, 1, 5 and 10 days old worms. (D) Relative mRNA levels of the BiFC2 construct in BiFC-syn worms and control worms expressing only the BiFC2 construct in L4, 1, 5 and 10 days old worms. Statistical significance was determined from the mean values of three independent qPCR experiments using ANOVA in conjunction with a Tukey’s post-hoc multiple comparisons test (alpha = 0.05). In all cases data is represented as mean of 3 independent experiment, represented as mean ± SEM. See also supplementary video 2.
