Figure 2 | Scientific Reports

Figure 2

From: Extreme anoxia tolerance in crucian carp and goldfish through neofunctionalization of duplicated genes creating a new ethanol-producing pyruvate decarboxylase pathway

Figure 2

Phosphorylation of crucian carp E1α subunits in normoxia (N7) and anoxia (A7). (A,B) Phosphorylation of E1α subunits in relation to N7 (n = 7) are shown for red muscle (A) and brain (B). Western blot images include staining using antibodies against phosphorylated PDHc (upper three images in A and image in B) and against total E1α (lower image in A). Statistical difference compared to N7 is indicated by * (p < 0.0001). n.s = not significant; kD = kilodalton. (C,D) EM micrographs of crucian carp red muscle showing gold-staining of phosphorylated E1α subunits during normoxia (C) and anoxia (D). Mit. = mitochondria; I/M = visible bands of muscle fibers. (E) Quantification of gold particles per µm2 in red muscle following N7, A1 and A7 (Means ± S.E.M. of 3 individuals per group). (See legend to Fig. 1).

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