Figure 5
Conservation of expression patterns of over-expressed silk transcripts (OESTs) among cobweb weaving spider species. (a) OESTs with homologs in all three spider species were identified by clustering transcripts with BlastClust30 and are referred to as BlastClust 3-species clusters. Venn Diagram indicates BlastClust 3-species clusters that contained an OEST from just one species, two species, or all three species. (b) Proportion of total mean FPKM in each of the silk gland and non-silk (NS) tissues attributed to OESTs in BlastClust 3-species clusters (colors), OESTs with no homologs in the other two species (dark gray), and OESTs with a homolog in only one other species (light gray). (c and d) Heat maps of pairwise Spearman’s correlation coefficients based on the summed mean FPKM of BlastClust 3-species clusters containing OESTs between each tissue type sampled from the three focal species. Correlation coefficients were used for heirarchical clustering (bootstrap proportions ≥50% shown). In (c) all OEST-containing clusters are included. In (d) the clusters containing known silk structural protein-encoding transcripts have been excluded. Tissue and gland abbreviations, and sample sizes, as in Figs 1 and 3.
