Table 1 Gene primer sequences and amplicon characteristics.
Gene symbol | Gene name | Primer sequence (5′–3′) | Product length (bp) | Tm (°C) | E (%) | R 2 |
|---|---|---|---|---|---|---|
α-TUB | alpha tubulin | F: GGTCAGGCTGGTGTTCAAAT | 173 | 60 | 102.77 | 0.996 |
R: ACGATGACCACTCTGGGAAC | ||||||
CycA | cyclin A | F: TTCGAGTGCATGAAGACCAG | 193 | 60 | 106.76 | 0.999 |
R: TTGCGTTTACGGCTCTCTTT | ||||||
EF1α | elongation factor 1α | F: CCAGGCATGGTAGTGACCTT | 184 | 60 | 98.05 | 0.997 |
R: TTGGAGGGTTGTTTTTGGAG | ||||||
γ-TUB | gamma tubulin | F: TGATATTTGGGAAGGCAAGG | 201 | 60 | 103.28 | 0.993 |
R: CGCCCAAGAGCGTAATAATC | ||||||
GAPDH | glyceraldehyde 3-phosphate dehydrogenase | F: GTTCCGATGTTTGTGTGTGG | 230 | 60 | 101.64 | 0.993 |
R: TTCTGCAATCCCGATCTACC | ||||||
RPL13a | ribosomal protein L13a | F: GCTCGTGGTCCATTTCATTT | 240 | 60 | 103.71 | 0.998 |
R: TCCCACTTCATGTGACAAGC | ||||||
RPS13 | ribosomal protein S13 | F: ATCCGTAAGCATTTGGAACG | 162 | 60 | 102.48 | 0.999 |
R: AGCCACTAAGGCTGAAGCTG | ||||||
SDA | succinate dehydrogenase complex subunit A | F: GCCAATTCCTGTTCTTCCAA | 155 | 60 | 94.71 | 0.994 |
R: CTCCGTGAACTGAAGCACAA | ||||||
β-ACT | beta actin | F: CCTCTTCCAGCCTTCCTTCT | 248 | 60 | 98.63 | 0.997 |
R: CACCGATCCAGACGGAGTAT | ||||||
nad3 | NADH dehydrogenase subunit 3 | F: GCCTTTTGAATGTGGATTTGA | 119 | 60 | 97.11 | 0.998 |
R: TTGGGAATAAAAGGGTAATTTCT | ||||||
atp6 | ATP synthase F0 subunit 6 | F: TTTCTAGGATTATTCCCCTATATTTTT | 129 | 60 | 109.60 | 0.999 |
R: GGCTAATATATGGGTTGTATTATTGAT | ||||||
cob | Cytochrome b | F: AAAATTTTTAAGAACACAATTCTACCC | 100 | 60 | 104.75 | 0.998 |
R: AACAGGGCGAGCTCCAAT | ||||||
cox1 | Cytochrome c oxidase subunit I | F: TACCGGGGTTGTTCTAGCTG | 232 | 60 | 110.06 | 0.993 |
R: AAAATGTTGGGGGAAAAAGG |
