Figure 6

ILDR2 affects TUBD1 and IQCB1 pre-mRNA splicing. (A) RT-PCR revealed that exon 4 of endogenous TUBD1 in HEK293T cells is subjected to alternative splicing (lane 1). The inclusion of exon 4 was enhanced by TRA2B (lane 2), whose effect was inhibited by ILDR1 (lane 3) or ILDR2 (lane 4), but not LSR (lane 5). The level of overexpression was examined via RT-PCR and Western blot. (B) RT-PCR revealed that exon 12 of endogenous IQCB1 in HEK293T cells is subjected to alternative splicing (lane 1). The inclusion of exon 12 was enhanced by SRSF1 (lane 2), whose effect was inhibited by ILDR1 (lane 3) or ILDR2 (lane 4), but not LSR (lane 5). The level of overexpression was examined via RT-PCR and Western blot. (C) RT-PCR revealed that the inclusion of exon 4 of TUBD1 in HEK293T cells was enhanced when ILDR1 and/or IDLR2 were knockdown. (D) RT-PCR revealed that the inclusion of exon 12 of IQCB1 in HEK293T cells was enhanced when ILDR1 and/or IDLR2 were knockdown. The relative exon inclusion rate was calculated from three independently performed experiments. The differences between groups were determined by Student’s t-test. **P < 0.01; ***P < 0.001; ns, not significant.