Figure 5 | Scientific Reports

Figure 5

From: Reactivity of human AGO2 monoclonal antibody 11A9 with the SWI/SNF complex: A case study for rigorously defining antibody selectivity

Figure 5

The 11A9 antibody enriches SWI/SNF factors in the absence of AGO2. (A,B) Label-free quantification of 11A9 antibody associated proteins compared to IgG isotype control. IP-MS experiments were performed in triplicate in wild type and AGO2 knock-out HEK293T cells. For the analysis a two-sample t-test was applied. (C) Table with mean Maxquant derived label free quantification (LFQ) intensities (x10) of 3 IP-MS experiments for 11A9 and IgG with HEK293T wild-type and AGO2 knock-out lysates. AGO2 TRNC6A, B and C signals in AGO2 knock-out extracts are the result form imputation. CV represents coefficient of variation. (D) ChIP-qPCR in wild type and knock-out HEK293T cells. The control region (heterochromatic site) and Meis1 locus represent negative sites.

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