Figure 2
BPA upregulated APP, BACE-1 and Aβ1–42 expressions. Western blot analysis of the expression of (A) APP protein in SY5Y cells treated with different concentrations of BPA (0, 2, 20, 200, and 2000 nM/L) at various time points (0, 1, 3, 6, 12 and 24 h). (C) APP and BACE-1 protein expressions in PC-12 cells at different concentrations of BPA (0, 2, 20, 200, and 2000 nM/L) at various time points (0, 1, 3, 6, 12 and 24 h). (B,D,F) GAPDH levels were assessed in parallel and served as controls. Data are presented as the mean ± SEM from three independent experiments. (E) Effect of different concentrations of BPA (0, 2, 20, 200, and 2000 nM/L) on BACE-1 expression in PC-12 cells. (G) Immunofluorescence analysis of the expression of BACE-1 protein after treatment with 20 nM/L BPA. (H,I) Effects of different concentrations of BPA on Aβ1–42 expression in PC-12 cells determined via ELISA. Mean values ± SEMs are representative of three independent isolations and three independent samples. Significant differences between the treatment groups and the control group were determined via one-way ANOVA and the Dunnett multiple comparison procedure. (*P < 0.05, **P < 0.01, ***P < 0.001 compared with the control group).
