Figure 3
BPA enhanced the expression of phosphorylated tau. Western blot analysis indicated the expression of phosphorylated tau in SY5Y cells treated with 20 nM/L BPA (A) at different concentrations (0, 2, 20, 200, and 2000 nM/L) and various time points (0, 1, 3, 6, 12, and 24 h). (C) Expression of phosphorylated tau in PC-12 cells treated with 20 nM/L BPA at various time points (0, 1, 3, 6, 12, and 24 h) of BPA. (E) Expression of phosphorylated tau in PC-12 cells treated with different concentrations of BPA (0, 2, 20, 200, and 2000 nM/L). (D,F) GAPDH levels were detected in parallel and served as controls. (G) Immunofluorescence analysis of the expression of p-tau after treatment with 20 nM/L BPA. Mean values ± SEMs are representative of three independent isolations and three independent samples. Significant differences between the treatment groups and the control group were determined via one-way ANOVA and the Dunnett multiple comparison procedure. (*P < 0.05, **P < 0.01, ***P < 0.001 compared with the control group).
