Figure 5
Effects of rosiglitazone and insulin on BPA-induced insulin signaling pathways. (A) Effects of rosiglitazone on the expression of IR and IRS tyrosine phosphorylation mediated by BPA in SY5Y cells. (C) Effects of rosiglitazone on the expression of AKT and GSK3α/3β serine phosphorylation mediated by BPA in SY5Y cells. (E) Effects of insulin on the expression of phosphorylated IR and phosphorylated IRS mediated by BPA in SY5Y cells. (G) Effects of insulin on the expression of phosphorylated AKT and GSK3α/3β mediated by BPA in SY5Y cells. (B,D,F,H) GAPDH levels were assessed in parallel and served as controls. Mean values ± SEMs are representative of three independent isolations and three independent samples. Significant differences between the treatment groups and the control group were determined via one-way ANOVA and the Dunnett multiple comparison procedure. (*P < 0.05, **P < 0.01 compared with the BPA single treatment group, #P < 0.05, ##P < 0.01).
