Figure 2
From: Efficient functional neutralization of lethal peptide toxins in vivo by oligonucleotides
In vitro evaluation of aptamer neutralization properties. (a) Dose-response curve of αC-conotoxin PrXA-mediated inhibition of 1 nM [125I]-α-BgTx binding. (b) Effect of D7 aptamer on αC-conotoxin PrXA-mediated inhibition of [125I]-α-BgTx binding. (c) Effect of B4 aptamer on αC-conotoxin PrXA-mediated inhibition of [125I]-α-BgTx binding. (d) Time-dependent inhibition of muscle contraction by 0.31 µM αC-conotoxin PrXA (left inset). Lack of toxin effect on direct muscle stimulation (right inset). (e) αC-conotoxin PrXA dose-dependence of muscle contraction inhibition. IC50 = 23 nM. (f) Effect of D7 and B4 aptamers on 28 nM αC-conotoxin PrXA-mediated inhibition of muscle contraction. D7 at 20x molar, B4 at 45x molar concentration. (g) Waglerin 1 dose-dependence of muscle contraction inhibition. (h) Effect of D7 aptamer on waglerin 1-mediated inhibition of muscle contraction. N.S.: nonspecific; ***: p ≤ 0.001.
