Figure 1
From: Fabrication of a protein microarray by fluorous-fluorous interactions
Protein modification strategies. (a) Combination of the intein expression system and NCL for the C-terminal modification of enhanced green fluorescent protein (eGFP), maltose binding protein (MBP), and glutathione transferase (GST) with fluorous tags. (b) An anti-Ricinus communis agglutinin 120 (anti-RCA120) antibody was modified with a fluorous tag by forming a boronate ester with a BA-containing fluorous tag. (c) Fluorous probes 1 and 2 were used for labeling in this study. Probes 3 and 4 were not used because of their poor solubility in water. Probe 5 was used for the purification of fluorous proteins.
