Figure 3
Photo-excited MB restores the phenotypes of Aβ42 toxicity in vivo model system. (a–h) The images of the NMJ boutons on muscle 6/7 of A3. Indicated genotype flies were incubated with or without 100 nM MB treatment under dark and red LED light. NMJ boutons were observed by HRP immunostaining. (a,c) NMJ of the Mhc-GAL4/+ control and (e,g) NMJ of the Mhc > Aβ42 under dark condition; (b,d) NMJ of the Mhc-GAL4/+ control and (f,h) NMJ of the Mhc > Aβ42 treated with or without 100 nM MB under red LED light. (h) NMJ morphology phenotype caused by Aβ42 overexpression is rescued by photoexcited-MB. Scale bar: 10 μm. (i) Effect of various concentration of MB on the total number of NMJ boutons on muscle 6/7 of A3. Indicated genotype flies were incubated with 0, 1, 10, and 100 nM concentration of MB under dark and red LED light conditions. (j,k) The diagram of the crawling path of the larvae on the plate. Diameter of inner-circles are 1.0 cm, 2.0 cm and 3.0 cm, respectively. (j) The crawling path of the Mhc-GAL4/+ control and (k) Mhc > Aβ42 with or without 100 nM MB treatment under dark and red LED light. The locomotor phenotype in Aβ42 overexpression is rescued by photoexcited MB. Scale bar: 2 cm (l) Quantification of crawled distance of larvae within 90 seconds. Indicated genotype flies were incubated with 0, 1, 10, and 100 nM concentration of MB under dark and red LED light conditions.
