Figure 3

4T07 and 4T1 Tumors Increase Liver Inflammation and Downstream IL-6 Signaling. (A–D) Liver cytokine mRNA expression of IL-1β, IL-6, TNFα, and IFNγ expressed as fold change from vehicle.(A–C) Liver IL-1β, IL-6, and TNFα were significantly increased in 4T07 and 4T1 tumors relative to 67NR tumors and vehicles. (D) There were no significant differences in liver IFNγ. (E–F) mRNA levels of downstream IL-6 signaling molecules, STAT3 and SOCS3 expressed as fold change from vehicle. (E) STAT3 mRNA expression was increased in 4T1 tumors relative to vehicles. (F) Liver Socs3 mRNA was significantly higher in 4T07 and 4T1 tumors relative to 67NR tumors and vehicles. The measures and tissues were collected 20 days after tumor cell or vehicle inoculation (n = 12–15/group). The data are presented as mean + SEM. Graph bars that do not share a letter are statistically significant different at p < 0.05.