Figure 4

Verteporfin (VP) activates p38 MAPK glioblastoma cells. SNB19 and LN229 cells were treated with vehicle (C), 2 µg/ml of VP (L) or 10 µg/ml of VP (H) for 24 hours protected from light at all steps. Western blot of protein extracts showed that VP treatment induced phosphorylation of p38 MAPK in a dose-dependent manner. Cropped blots are shown. Quantification of the blots relative to Coomassie blue staining, as a loading control, is on the right. (Experiment repeated 3 times, representative blot is shown here).