Figure 3
RAGE inhibition decreases lung epithelial injury. Levels of (A) bronchoalveolar fluid lavage (BAL), (B) plasma sRAGE, (C) lung RAGE mRNA, and (D) protein expression in the uninjured, untreated mice (Sham group), acid-injured animals (HCl group), acid-injured animals treated with sRAGE (HCl + recombinant sRAGE group) or with anti-RAGE monoclonal antibody (HCl + anti-RAGE mAb group) (n = 4–6 for each time-point). Levels of sRAGE and RAGE proteins were measured in duplicate via ELISA. Levels of BAL and plasma sRAGE were normalised to those of total protein. Levels of mRNA expression (∆∆Ct) were normalised to housekeeping genes. Protein and mRNA expression levels are expressed as ratios to those in sham controls. As no difference was observed between sham animals at all time points; the results from sham mice were mixed for analyses (left bar of the X-axis). Values are reported as means ± standard deviations. *P < 0.05; **P < 10−2 versus sham controls; #P < 0.05, ##P < 10−2 versus acid-injured animals.
