Figure 4
From: Oleate but not stearate induces the regulatory phenotype of myeloid suppressor cells
Kinetic of lipid droplets formation and suppressive capacity after sodium oleate treatment. MSC-2 cells were cultured in the presence or absence of sodium oleate (0.2 mM) for 24 h. Cells were washed and left in BSA-medium for up to 72 h and the analysis was performed at the time points indicated. The formation of lipid droplets (A) were analyzed by BODIPY staining. The regulatory function of MSC-2 cells from those groups was analyzed via T cell proliferation assay (B). Supernatant from the co-culture served for the quantification of NO production (C). Shown is the mean ± SD from two to three independent experiments. ***p ≤ 0.001.
