Figure 1 | Scientific Reports

Figure 1

From: Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore

Figure 1The alternative text for this image may have been generated using AI.

Ca2+-depletion reduces the stability of spindle fibres. (a) HeLaWT cells arrested at metaphase using MG132 were treated with 25 µM BAPTA-AM or 10 mM BAPTA and 5 µM ionomycin to reduce intracellular Ca2+ then stained with anti-Ac-tubulin antibody. (b) A graph presenting the relative Ac-tubulin intensity at kinetochore of each treatment. (c) Fluorescence images of metaphase cells in control, BAPTA-AM and BAPTA (with ionomycin) treatments after exposure to low temperature. (d) A graph presenting the relative cold-stable microtubule intensity at kinetochore of each treatment. (e) A bar graph showing the intensity ratio of Fura-2 excited at 340 and 380 nm measured in mitotic cells, which represent intracellular calcium levels. Error bars indicate standard deviations derived from three independent experiments. Bar, 5 µm.

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