Figure 1

Transplantation of a bio-implant in a tooth extraction model and characteristics of the immortalized human cells. (a) Schematic representation of the bio-implant procedure. The main purpose of this study is periodontium regeneration around implant fixture. (b) Combination of cell sheets used in this study. Various types of cell sheet (panel a) are described. (c) Maxillary first molar and HA-coated dental implant fixture. (d) Micro CT image of 8 weeks after transplantation of the HA-coated fixture shows the alveolar bone compactly healed around the fixture. (e) 3D reconstruction of the micro CT results at 8 weeks after transplantation of the HA-coated fixture. (f) HE-stained image at 8 weeks after transplantation of the HA-coated fixture showing osseointegration between the alveolar bone and the fixture. Black dashed line shows the margin of the removed implant fixture. (g) RT-PCR results of immortalized human cells (cropped gel image). The ihCEMs expressed ALP, BSP and CEMP-1. The ihPDLs expressed PLAP-1 and ColI. Full-length gel is presented in Supplementary Figure 6. Scale bars, (b,c) 1 mm; (e) 200 μm.