Figure 2
Periodontium-like tissue formation by transplanted cell sheets. (a) HE-stained image at 8 weeks after ihPDLs sheet transplantation. PDL-like connective tissue is observed between the fixture and the alveolar bone. (b) High magnification of panel a. The alignment of the PDL-like tissue is parallel to the fixture. (c,d) IHC of the PDL markers, periostin and fibrillin 1. The periostin and fibrillin 1 staining was positive around the PDL-like tissue. (e) IHC of the blood vessel marker, vWF. Blood vessels were observed in the alveolar bone and PDL-like tissue. (f) IHC of HLA. The PDL-like tissue showed a strong HLA signal, and the signal was also observed in some areas of the alveolar bone. (g) IHC of GFP. In the ihPDLs sheet transplantation, GFP was not detected. (h) Resorcin-fuchsin staining. Arrows show the oxytalan fibres in the PDL-like connective tissue. (i) HE-stained image at 8 weeks after ihCEM [HUVEC]/ihPDL bi-layered cell sheet transplantation. (j) High magnification of panel g. The arrows indicate remodelled bone. The alignment of the fibrous tissue is perpendicular to the fixture. (k,l) IHC of periostin and fibrillin 1. Positive periostin and fibrillin 1 signalling were detected in the PDL-like tissue. (m) IHC of vWF. Blood vessels were observed around the remodelled bone and in the PDL-like tissue. (n) IHC of HLA. The PDL-like tissue shows positive HLA signalling. (o) IHC of GFP. This shows blood vessels in the PDL-like tissue originated from the GFP tagged HUVECs. (p) Resorcin-fuchsin staining. Arrows indicate the oxytalan fibres in the PDL-like tissue. Scale bars, (a,i) 500 μm; (b–h,j–p) 100 μm.
