Figure 4
From: Optimised metrics for CRISPR-KO screens with second-generation gRNA libraries
Optimal number of gRNAs per gene and number of replicates. (a) Box plots showing area under the curve (AUC) from ROC analysis using different gRNAs per gene. (b) The number of depleted genes detected at the indicated FDR and fold change (FC) cut-offs. (c) The number of depleted genes detected at FDR ≤ 10% and FC ≤ 0.5 from reduced numbers of replicates for each subset.
