Figure 2
From: Identification of the novel 3′ UTR sequences of human IL-21 mRNA as potential targets of miRNAs
Long 3′ UTR of human IL-21 mRNA was identified from human T cells. (A) Relative expression level of IL-21 mRNA was examined by quantitative real-time RT-PCR. Data shown are a representative of three independent experiments performed. Bars indicate the means of triplicates. (B) RT-PCR was performed with RNA from human Th1 cells to detect human IL-21 mRNA. Revere primers were designed in putative regions of 3′ UTR of human IL-21 mRNA. One representative data of three experiments is shown. (C) The 3′ end of human IL-21 mRNA was identified by the 3′ RACE experiment. The forward primer was designed to anneal to the 2463–2484 nt of the 3′ UTR. Asterisk indicates a non-specific band. (D) The identified 3′ UTR of human IL-21 mRNA and its conserved target sites of the EV-miRNAs are illustrated.
