Figure 1

Frequency of CRISPR/Cas9-induced mutation types. (A,B) Frequency of each mutation type for all of the mutations induced by the 10 constructs in the T0 generation. i: insertion; d: deletion; s: substitution; c: combined mutation. d#, number of base pairs (bp) deleted from the target site; i#, number of bp inserted at target site, c#, number of bp combined mutations. (C) Frequency of different mutation lengths regardless of the mutation types using the data from (A). (D) Percentage of different bases for the 1-bp insertion (i1 in (B)). (E) Detailed characterization of the different mutation types. Notes: i: insertion, d: deletion, s: substitution, c: combined mutation (more than one mutation type in one allele).