Figure 2 | Scientific Reports

Figure 2

From: CRISPR/Cas9-mediated genome editing efficiently creates specific mutations at multiple loci using one sgRNA in Brassica napus

Figure 2

Genotyping of the BnaC5.DA1-sgRNA-L16 plants in the T0 and T1 generation. (A) Cropped gel image showing the PCR products derived from Cas9 in BnaC5.DA1-sgRNA-L16 in the T0 generation. (B) The genotype of BnaC5.DA1-sgRNA-L16 in the T0 generation. The PAM sequence is indicated with green. The sgRNA is indicated with red. Mutation sites are indicated with blue. (C) Cropped gel image showing the PCR products of Cas9 in different progeny from BnaC5.DA1-sgRNA-L16 in the T1 generation. +: pKSE401 was used as a positive control; −: gDNA of WT was used as a negative control. BnaC5.DA1-sgRNA-L1 (T0 and T1) was used as a positive control for the Cas9 insertion. The arrowheads indicate the position of the amplicons.

Back to article page